Theory of operating HPLC column in reversed flow direction

Many operators try to reverse the flow direction of HPLC column to obtain acceptable system suitability results such as ( Tailing factor, Plate count and resolution )

- In your opinion, Do you think that this actually affects on system suitability ?

- What is the scientific theory of this ?

- Do you think that this will affect on the column performance on the long run 🤔? 





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