Theory of operating HPLC column in reversed flow direction
Many operators try to reverse the flow direction of HPLC column to obtain acceptable system suitability results such as ( Tailing factor, Plate count and resolution )
- In your opinion, Do you think that this actually affects on system suitability ?
- What is the scientific theory of this ?
- Do you think that this will affect on the column performance on the long run 🤔?
" Lets discuss this with each other in the comments 👇"
Yes,
ReplyDeleteIf it is repeated several times;it will affect the column performance.. for sure
ReplyDeleteYes sure 👍
DeleteYes sure 👍
DeleteShedulSc for after 300 injection complete on column performance....
Delete