1- C8 : Has only 8 carbon atoms chain bonded to silica atom, less dense than C18 (Decreases the surface area) so the elution will be faster (Decreases retention time). 2- C18 : Has only 18 carbon atoms chain bonded to silica atom, more dense than C8 (Increases the surface area) so the elution will be slower (Increases retention time).

 

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