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Reversed phase vs Normal phase
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How to calculate peak system suitability
1- Asymmetry factor 2- Tailing factor 3- Theoretical plate count 4- Resolution 5- Peak to valley ratio 6- Signal to noise ratio
Effect of Acetonitrile, Methanol & Water ratio on retention time
1- Water : Acetonitrile (45 : 55) 2- Water : Methanol (45 : 55) 3- Water : Methanol (32.2 : 67.8) 4- A) Organic : Buffer (3 : 2) B) Organic : Buffer (3 : 2) 5- Methanol : Water (80 : 20) 6- Methanol : Water (70 : 30) 7- A) Acetonitrile : Water (30 : 70) B) Acetonitrile : Methanol : Water (15 : 15 : 70) C) Acetonitrile : THF : Water (15 : 15 : 70)
I think front tailing is usually due to the solvent effect try either to minimize the injection volume or to change the last dilution of test to be in mobile phase and not in diluent
ReplyDeleteYes I totally agree with you, also it may be because the column loses its efficiency
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