Determination of melamine in Liquid & Powder milk by HPLC

1- Mobile phase preparation :

- Eluent (A) :10 mM Acetic acid + 10 mM Ammonium acetate, pH 4.3 (70:30 v/v)

- Eluent (B) : Acetonitrile

- Eluent (A) : Eluent (B)

            80   :   20

2- Column : Acclaim Mixed-Mode WCX-1

( 25 cm x 4.6 mm x 5 µm )

3- Column oven temp. : 30 °C

4- Detector : UV 240nm

5- Injection volume : 20 µL

6 - Chromatogram :

- Peak 1 : Melamine