Factors affect on retention time of peak


 

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Theory of operating HPLC column in reversed flow direction

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Many operators try to reverse the flow direction of HPLC column to obtain acceptable system suitability results such as ( Tailing factor, Plate count and resolution ) - In your opinion, Do you think that this actually affects on system suitability ? - What is the scientific theory of this ? - Do you think that this will affect on the column performance on the long run 🤔?  " Lets discuss this with each other in the comments 👇"
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