Factors affect on retention time of peak
Reversed phase vs Normal phase
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1- Asymmetry factor 2- Tailing factor 3- Theoretical plate count 4- Resolution 5- Peak to valley ratio 6- Signal to noise ratio
Effect of Acetonitrile, Methanol & Water ratio on retention time
1- Water : Acetonitrile (45 : 55) 2- Water : Methanol (45 : 55) 3- Water : Methanol (32.2 : 67.8) 4- A) Organic : Buffer (3 : 2) B) Organic : Buffer (3 : 2) 5- Methanol : Water (80 : 20) 6- Methanol : Water (70 : 30) 7- A) Acetonitrile : Water (30 : 70) B) Acetonitrile : Methanol : Water (15 : 15 : 70) C) Acetonitrile : THF : Water (15 : 15 : 70)
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