How to back flush HPLC column
Theory of operating HPLC column in reversed flow direction
Many operators try to reverse the flow direction of HPLC column to obtain acceptable system suitability results such as ( Tailing factor, Plate count and resolution ) - In your opinion, Do you think that this actually affects on system suitability ? - What is the scientific theory of this ? - Do you think that this will affect on the column performance on the long run 🤔? " Lets discuss this with each other in the comments 👇"
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