USP HPLC columns

 


USP HPLC columns list 👇

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Theory of operating HPLC column in reversed flow direction

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Many operators try to reverse the flow direction of HPLC column to obtain acceptable system suitability results such as ( Tailing factor, Plate count and resolution ) - In your opinion, Do you think that this actually affects on system suitability ? - What is the scientific theory of this ? - Do you think that this will affect on the column performance on the long run 🤔?  " Lets discuss this with each other in the comments 👇"
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Effect of Acetonitrile, Methanol & Water ratio on retention time

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  1- Water : Acetonitrile (45 : 55) 2- Water : Methanol (45 : 55) 3- Water : Methanol (32.2 : 67.8) 4- A)  Organic : Buffer (3 : 2)      B) Organic : Buffer (3 : 2) 5- Methanol : Water (80 : 20) 6- Methanol : Water (70 : 30) 7-  A) Acetonitrile : Water (30 : 70)       B) Acetonitrile : Methanol : Water                             (15 : 15 : 70)       C) Acetonitrile : THF : Water                             (15 : 15 : 70)
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