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 1- C8 : Has only 8 carbon atoms chain bonded to silica atom, less dense than C18 (Decreases the surface area) so the elution will be faster (Decreases retention time). 2- C18 : Has only 18 carbon atoms chain bonded to silica atom, more dense than C8 (Increases the surface area) so the elution will be slower (Increases retention time).

 

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  1- Mobile phase preparation : - Eluent (A) : Methanol - Eluent (B) : Water - Eluent (A) : Eluent (B)             70   :   30 2- Column :   Acclaim 120 C18 ( 25 cm x 4.6 mm x 5  µm ) 3- Column oven temp. :   25   °C 4- Detector :   UV 220nm 5- Injection volume :  1 0 µL 6 - Chromatogram : Peak 2 : Ethinylestradiol

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1- Mobile phase preparation : - Eluent (A) :10 mM  Acetic acid + 10 mM Ammonium acetate, pH 4.3 (70:30 v/v) - Eluent (B) : A cetonitrile - Eluent (A) : Eluent (B)             80   :   20 2- Column :   Acclaim Mixed-Mode WCX-1 ( 25 cm x 4.6 mm x 5  µm ) 3- Column oven temp. :   30 °C 4- Detector :   UV 240nm 5- Injection volume :   20 µL 6 - Chromatogram : - Peak 1 : Melamine

 

 

 

 

Many operators try to reverse the flow direction of HPLC column to obtain acceptable system suitability results such as ( Tailing factor, Plate count and resolution ) - In your opinion, Do you think that this actually affects on system suitability ? - What is the scientific theory of this ? - Do you think that this will affect on the column performance on the long run 🤔?  " Lets discuss this with each other in the comments 👇"

 

 

  1- Mobile phase preparation : - Eluent (A) :  potassium dihydrogen phosphate, 25 mM, pH 2.85 - Eluent (B) : A cetonitrile - Eluent (A) : Eluent (B)             25   :   75 2- Column :   Acclaim Mixed-Mode HILIC-1 ( 25 cm x 4.6 mm x 5  µm ) 3- Column oven temp. :   30 °C 4- Detector :   UV 200nm 5- Injection volume :   20 µL 6 - Chromatogram : - Peak 1 : Glutamic acid - Peak 2 : Arginine - Peak 3 : Impurity